Current eDNA sampling technologies consist mainly of do‐it‐yourself solutions. The lack of purpose‐built sampling equipment is limiting the efficiency and standardization of eDNA studies. So, Thomas et al. (a team of molecular ecologists and engineers) designed ANDe™.
In this video, the authors highlight the key features and benefits of ANDe™. This integrated system includes a backpack-portable pump that integrates sensor feedback, a pole extension with remote pump controller, custom‐made filter housings in single‐use packets for each sampling site and on-board sample storage.
“My research is broadly focussed on the evolution of complexity. Many of my projects are related to the evolutionary ecology of programmed cell death (PCD) in unicellular organisms; how PCD impacts microbial communities; and how the philosophy of levels of selection informs our understanding of PCD evolution. I have also examined other aspects of complexity evolution such as the origin of life and group formation in unicellular chlorophytes in response to predation. The model organisms I typically use are phytoplankton. With specific reference to submissions to Methods in Ecology and Evolution, I have used a range of methods in my research, including general cell and molecular biology tools, biochemical assays, microscopy, flow cytometry, bioinformatics and computational algorithms.”
“I’m a molecular ecologist who uses genetic and genomic tools to ask questions ranging from surveillance and monitoring to biodiversity and phylogeography. My work includes development of novel molecular detection tools and metabarcoding applications for aquatic invasive species. I’m also interested in applying molecular tools to ask questions related to the evolution and biodiversity of benthic marine invertebrates in Antarctica.”
It’s not easy to characterise the local environment of species living in mountains because these habitats are highly heterogeneous. At a large scale, we typically assume that temperature varies with altitude, but at a local scale, we understand that exposure to wind or being in the shade has a great influence on climatic conditions. If you go from the south-facing to the north-facing side of a mountain, it can be easily 5°C colder. If we can feel that, so can the organisms that live up there. Plants in particular are submitted to tremendous climatic variations over a year. What we want to know is: how did they adapt to these climatic variations and how localised is their adaptation?
Overcoming the Challenges of Measuring Local Adaptation
We don’t know much about how organisms adapt locally because it’s so difficult to measure the environmental conditions that these plants are facing. Existing weather stations can’t capture micro-habitat conditions because they are few and far between. What we can do instead, is use topographic models of mountains to model their environment. After all, if orientation, slope or shade have an impact on climatic conditions, why couldn’t we use them to model local variations in temperature for example? Continue reading →