Using Dual RNA-seq to Investigate Host-Pathogen Systems When Genomic Resources are Limited

Post provided by KAYLEIGH O’KEEFFE

Tall fescue infected with fungal pathogen, Rhizoctonia solani. ©Brooklynn Newberry

Tall fescue infected with fungal pathogen, Rhizoctonia solani. ©Brooklynn Newberry

Pathogens and the infectious diseases that they cause can have devastating impacts on host individuals and populations. To better understand how pathogens are able to cause disease, we can investigate the genetic mechanisms underlying the infection process. Hosts may respond to infection by upregulating defence pathways. Pathogens, in turn, evade these host immune responses as they infect and cause disease. As this process unfolds and each organism responds to the other, gene expression changes in both the host and the pathogen. These gene expression changes can be captured by dual RNA‐seq, which simultaneously captures the gene expression profiles of a host and of a pathogen during infection. Continue reading