The use of molecular methods for monitoring and surveillance of organisms in aquatic and marine systems has become more and more common. We’ve since expanded this technology this through using both captured whole organisms and collecting/filtering environmental DNA (eDNA). These methods naturally migrated from single species, active surveillance methods towards using high throughput sequencing as a method of passive surveillance via metabarcoding.
In this virtual issue, the article “Assessing strengths and weaknesses of DNA metabarcoding-based macroinvertebrate identification for routine stream monitoring” by Vasco Elbrecht et al. provides an excellent overview to the field. It also helps to clarify the work being done to provide interested groups, including management agencies, with the best practices for utilising these new methods for monitoring and surveillance. This work will help the field, particularly for those searching for rare species of organisms in aquatic systems.
I’d recommend this paper to all researchers and management groups interested in applying metabarcoding techniques to answer both experimental and applied questions. The design of this article will provide both experienced researchers and those new to the field with important information to further this rapidly expanding field.
To find out more about, read the full Methods in Ecology and Evolution article ‘Assessing strengths and weaknesses of DNA metabarcoding-based macroinvertebrate identification for routine stream monitoring’
This article is part of ‘Practical Tools: A Field Methods Virtual Issue’. All articles in this Virtual Issue will be available for a limited time.