Editor Recommendation: Assessing Strengths and Weaknesses of DNA Metabarcoding-Based Macroinvertebrate Identification for Routine Stream Monitoring

Post provided by Andrew R. Mahon

The use of molecular methods for monitoring and surveillance of organisms in aquatic and marine systems has become more and more common. We’ve since expanded this technology this through using both captured whole organisms and collecting/filtering environmental DNA (eDNA).  These methods naturally migrated from single species, active surveillance methods towards using high throughput sequencing as a method of passive surveillance via metabarcoding.

In this virtual issue, the article “Assessing strengths and weaknesses of DNA metabarcoding-based macroinvertebrate identification for routine stream monitoring” by Vasco Elbrecht et al. provides an excellent overview to the field. It also helps to clarify the work being done to provide interested groups, including management agencies, with the best practices for utilising these new methods for monitoring and surveillance.  This work will help the field, particularly for those searching for rare species of organisms in aquatic systems.

I’d recommend this paper to all researchers and management groups interested in applying metabarcoding techniques to answer both experimental and applied questions. The design of this article will provide both experienced researchers and those new to the field with important information to further this rapidly expanding field.

To find out more about, read the full Methods in Ecology and Evolution article ‘Assessing strengths and weaknesses of DNA metabarcoding-based macroinvertebrate identification for routine stream monitoring

 This article is part of ‘Practical Tools: A Field Methods Virtual Issue’. All articles in this Virtual Issue will be available for a limited time.

Practical Tools: A New Article Type and a Virtual Issue

Today, we’re pleased to announce that we’re launching a new article type for Methods in Ecology and Evolution: Practical Tools. Like our Applications articles, Practical Tools will be short papers (up to 3000 words). They’ll focus on new field techniques, equipment or lab protocols. From this point forward, our Applications papers will solely focus on software and code.

Practical tools need to clearly demonstrate how tools designed for specific systems or problems can be adapted for more general use. Online supporting information can include specific instructions, especially for building equipment. You can find some examples of Applications that would now fit into this article type here and here.

To help launch our new article type, we asked four of our Associate Editors – Pierre Durand, Graziella Iossa, Nicolas Lecomte and Andrew Mahon – to put together a Virtual Issue of papers about Field Methods that have previously been published in the journal. All of the articles in ‘Practical Tools: A Field Methods Virtual Issue‘ will be free to everyone for the next month. You can find out a bit more about each of the four sections of the Virtual Issue below. Continue reading

Virtually Trekking Across the Pond with the Newest Senior Editor: Aaron M. Ellison

Post Provided by Aaron Ellison

I’m delighted to be the newest member of the diverse team of Senior and Associate Editors who have made Methods in Ecology and Evolution one of the premier journals in the field. After 15 years working on the lead editorial teams of Ecology and Ecological Monographs, I’m really looking forward to applying my editorial energies to the ESA’s friendly competitor on the other side of the ‘pond’.

My background includes:

  • an undergraduate degree in East Asian Philosophy
  • a PhD in evolutionary ecology
  • research and teaching on the natural history and population, community, and landscape ecology of plants and animals (mostly invertebrates) in the marine intertidal and subtidal, among salt marshes and mangroves, tropical and temperate forests, and carnivorous plant bogs
  • extensive forays into statistics, mathematics, and software engineering
  • increasing attention to the history and practice of art and architecture and their relationship to ecological theory
  • a quirky social-media persona
  • and more than two decades of work in editing and publishing journals with scientific societies.

All of these things contribute to my open, catholic approach to scientific research, teaching, and publishing, and their relationship to the broader world.

The editors of Methods are always interested in seeing papers on methodological advances and approaches that lead to new directions. We love reading about creative solutions for new challenges in ecological and evolutionary research and applications in the broadest sense. As a new Senior Editor, I’m especially hoping to encourage more papers in three areas: field methods (about which I’ve published two of my own papers in Methods), reproducibility, and science communication. Continue reading

A New Way to Study Bee Cognition in the Wild

Understanding how animals perceive, learn and remember stimuli is critical for understanding both how cognition is shaped by natural selection, and how ecological factors impact behaviour.Unfortunately, the limited number of protocols currently available for studying insect cognition has restricted research to a few commercially available bee species, in almost exclusively laboratory settings.
In a new video Felicity Muth describes a simple method she developed with Trenton Cooper, Rene Bonilla and Anne Leonard for testing both lab- and wild-caught bees for their preferences, learning and memory. They hope this method will be useful for students and researchers who have not worked on cognition in bees before. The video includes a tutorial for carrying out the method and describes the data presented in their Methods in Ecology and Evolution article, also titled ‘A novel protocol for studying bee cognition in the wild‘.

This video is based on the article ‘A novel protocol for studying bee cognition in the wild by Muth et al.

 

Senior Editor Vacancy at Methods in Ecology and Evolution

Issue 6.7_Kakadu FloodplainsThe British Ecological Society (BES) is a thriving learned society established in 1913 whose vision is a world inspired, informed and influenced by ecology. It publishes five successful journals, and a quarterly newsletter, the Bulletin, that is distributed to its 5,000 members worldwide. At present, the BES is seeking an outstanding ecologist to join the team of Senior Editors on Methods in Ecology and Evolution.

Methods in Ecology and Evolution (MEE) is a high-profile broad-scope journal which promotes the development of new methods in ecology and evolution and facilitates their dissemination and uptake by the research community. It brings together papers from previously disparate sub-disciplines to provide a single forum for tracking methodological developments in all areas. The journal has excellent citation metrics including a current Impact Factor of 6.34 and an active social media presence.

Submissions to MEE are growing and we are seeking an Senior Editor to strengthen and complement the editorial team and to continue raising the journal’s profile worldwide. The journal’s editorial team currently consists of three Senior Editors who are supported by an international board of around 60 Associate Editors and dedicated editorial office personnel. The Editors work together to determine journal strategy and to increase the reputation and quality of the journal, in addition to making decisions on around 800 manuscripts submitted each year. Further details about the Journal and its current editorial team can be found at www.methodsinecologyandevolution.org. Continue reading

What is Methods in Ecology and Evolution?

In a new Methods in Ecology and Evolution podcast, the Senior Editors – Rob Freckleton, Bob O’Hara and Jana Vamosi – discuss the past, present and future of the journal. They talk about what sets it apart from other journals, their favourite articles and the kinds of papers that they would like to see more of. If you’re thinking about submitting to Methods in Ecology and Evolution, they have some advice for you as well.

Articles Mentioned by the Editors:

To find out more about Methods in Ecology and Evolution, read our Aims and Scope and Author Guidelines

Scat Collection Protocols for Dietary DNA Metabarcoding

DNA dietary analysis is a non-invasive tool used to identify the food consumed by vertebrates. The method relies on identifying prey DNA in the target animals’ scats. It’s especially useful for marine animals such as seals and seabirds as it is difficult to watch their feeding events.

In the video below, Julie McInnes describes scat collection protocols that she (along with Rachael Alderman, Bruce Deagle, Mary-Anne Lea, Ben Raymond and Simon Jarman) developed to optimise the detection of food DNA in vertebrate scat samples. The authors use the shy albatross to demonstrate their new methods.

Continue reading

Testing the Effects of Underwater Noise on Aquatic Animals

Post provided by Karen de Jong

Most people assume that research equipment is expensive and complicated. But, it doesn’t need to be and the noise egg is a perfect example of this. It consists of a watertight container (as used by scuba divers) and the buzzer from a cellphone and does exactly what it says: it produces low frequency noise. This allows researchers to test the effect of noise on underwater life. It is a small, simple and cheap device that anyone can build.

Why Test Effects of Noise?

A painted goby in front of his nest ©K. de Jong

A painted goby in front of his nest ©K. de Jong

Underwater noise is rapidly increasing due to, for example, boat traffic and offshore wind farms. This can lead to stress for animals and difficulties in communication. Just as people have a hard time communicating in a noisy pub, animals may struggle to get their messages across when background noise is high. A nice description of how animals use sound and how noise may affect this can be found at www.dosits.org

While there is some knowledge on the effect of noise on large aquatic animals, we still know very little about how fish and other small aquatic animals are affected. Such knowledge is vital for management of protected areas. It’s also important to know whether wind farms and boat traffic can affect reproduction in populations of underwater resources such as fish and mussels. The answers to these questions are likely to be species specific, so we’ll need data on a large number of species in different habitats. Continue reading

Ecological Transcriptomics for Endangered Species: Avoiding the “Successful Operation, but the Patient Died” Problem

Post provided by TILL CZYPIONKA, DANIEL GOEDBLOED, ARNE NOLTE and LEON BLAUSTEIN

Ecological Transcriptomics and Endangered Species

 The small size of the rockpool and the salamander population makes non-invasive sampling a necessity (from left: Tamar Krugman, Alan Templeton, Leon Blaustein). © Arne Nolte

The small size of the rockpool and the salamander population makes non-invasive sampling a necessity (from left: Tamar Krugman, Alan Templeton, Leon Blaustein). © Arne Nolte

Friday was Endangered Species Day – so this is a good time to reflect on what science and scientists can do to support conservation efforts and to reduce the rate of species extinctions. One obvious answer is that we need to study endangered species to understand their habitat requirements as well as their potential for acclimatization and adaptation to changing environmental conditions. This information is crucial to for the design of informed conservation planning. However, for most endangered species the relevant phenotypes are not known a priori, which leaves the well-intentioned scientist asking “which traits should I measure?”. Transcriptome analysis is often a good way to answer to this question.

Transcriptome analysis measures the expression levels of thousands of genes in parallel. This amount of data circumvents the need to decide on a reduced number of traits of unknown relevance and allows for a relatively unbiased phenotypic screen of many traits. In particular, physiological changes, which often influence a species’ distributional range, can be studied using transcriptome analysis. Also, transcriptomics provide a direct connection to the genetic level. This is essential for in-depth analyses of aspects of evolution and might even be helpful for a new kind of conservation planning, which aims to foster endangered species by promoting (supposedly) beneficial hybridization. The integration of transcriptomic analysis with ecological studies is known as ‘Ecological transcriptomics’. Continue reading

Progress and Future Directions for Passive Acoustic Monitoring: Listening Out for New Conservation Opportunities

Post provided by Ammie Kalan (Post-doctoral researcher at the Max Planck Institute for Evolutionary Anthropology, Department of Primatology)

A Primate Call in a Forest is like a ‘Needle in a Haystack’

An ARU powered by solar energy recording in the Taï national park, Côte d’Ivoire. ©Ammie Kalan

A solar-powered ARU recording in the Taï national park, Côte d’Ivoire.
©Ammie Kalan

Finding a call of a particular primate species within hours and hours of audio recordings of a forest is no easy task; like finding a ‘needle in a haystack’ so to speak. Automated acoustic monitoring relies on the ability of researchers to easily locate and isolate acoustic signals produced by species of interest from all other sources of noise in the forest, i.e. the background noise. This can be much harder than it sounds. Think about whenever you have to use any kind of voice recognition system: seeking out a quiet room will greatly improve the chances you are understood by the robot-like voice on the other end of the phone. If you ever set foot in a rainforest the first thing you’ll notice is that it is anything but quiet. In fact characterizing and quantifying soundscapes has become a marker for the complexity of the biodiversity present in a given environment.

Primate monitoring programmes can learn a great deal from cetacean research where Passive Acoustic Monitoring (PAM) is the norm (since individuals are rarely observable visually). Research on bats and birds can provide excellent examples to follow as well. Automated algorithm approaches including machine learning techniques, spectral cross-correlation, Gaussian mixture models, and random forests have been used in these fields to be able to detect and classify audio recordings using a trained automated system. Such automated approaches are often investigated for a single species but impressive across-taxa efforts have also been initiated within a framework of real-time acoustic monitoring. Implementing these in other research fields could lead to significant advances. Continue reading